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Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
27/03/2023 |
Data da última atualização: |
12/04/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. |
Afiliação: |
PAULA CRISTINA DA SILVA ANGELO, CNPCa; L. F. P. PEREIRA, IDR - PARANÁ; G. H. SERA, IDR - PARANÁ; T. SERA, IDR - PARANÁ. |
Título: |
Coffee microspore cultivation to attain doubled-haploid plantlets. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Acta Horticulturae, v. 31, n. 1359, p. 123-130, 2023. |
DOI: |
10.17660/ActaHortic.2023.1359.14 |
Idioma: |
Inglês |
Notas: |
Apresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. |
Conteúdo: |
Introgression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation. MenosIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control ant... Mostrar Tudo |
Thesagro: |
Embriogénese; Pólen; Rubiaceae. |
Thesaurus Nal: |
Embryogenesis; Pollen. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02752naa a2200241 a 4500 001 2152776 005 2023-04-12 008 2023 bl uuuu u00u1 u #d 024 7 $a10.17660/ActaHortic.2023.1359.14$2DOI 100 1 $aANGELO, P. C. da S. 245 $aCoffee microspore cultivation to attain doubled-haploid plantlets.$h[electronic resource] 260 $c2023 500 $aApresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. 520 $aIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation. 650 $aEmbryogenesis 650 $aPollen 650 $aEmbriogénese 650 $aPólen 650 $aRubiaceae 700 1 $aPEREIRA, L. F. P. 700 1 $aSERA, G. H. 700 1 $aSETA, T. 773 $tActa Horticulturae$gv. 31, n. 1359, p. 123-130, 2023.
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Embrapa Café (CNPCa) |
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Registros recuperados : 22 | |
2. | | CAMPOS, G. S.; REIMANN, F. A.; CARDOSO, L. L.; FERREIRA, C. E. R.; JUNQUEIRA, V. S.; SCHMIDT, P. I.; BRACCINI NETO, J.; YOKOO, M. J. I.; SOLLERO, B. P.; BOLIGON, A. A.; CARDOSO, F. F. Genomic prediction using different estimation methodology, blending and cross-validation techniques for growth traits and visual scores in Hereford and Braford cattle. Journal of Animal Science, v.96, n. 7, p. 2579-2595, June 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sul. |
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3. | | CAMPOS, G. S.; REIMANN, F. A.; JUNQUEIRA, V. S.; BRACCINI, J.; CARDOSO, L. L.; YOKOO, M. J. I.; SOLLERO, B. P.; GULIAS GOMES, C. C.; BOLIGON, A. A.; CAETANO, A. R.; CARDOSO, F. F. Tag-SNP selection using Bayesian genome-wide association study for growth and adaptation traits in Hereford and Braford cattle. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland, New Zealand. Proceedings... Massey University, 2018.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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4. | | CAMPOS, G. S.; REIMANN, F. A.; JUNQUEIRA, V. S.; BRACCINI, J.; CARDOSO, L. L.; YOKOO, M. J. I.; SOLLERO, B. P.; GULIAS GOMES, C. C.; BOLIGON, A. A.; CAETANO, A. R.; CARDOSO, F. F. Tag-SNP selection using Bayesian genome-wide association study for growth and adaptation traits in Hereford and Braford cattle. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland, New Zealand. Proceedings... Massey University, 2018. Digital Archive.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sul. |
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5. | | CAMPOS, G. S.; SOLLERO, B. P.; REIMANN, F. A.; JUNQUEIRA, V. S.; CARDOSO, L. L.; YOKOO, M. J. I.; BOLIGON, A. A.; BRACCINI, J.; CARDOSO, F. F. Tag-SNP selection using Bayesian genomewide association study for growth traits in Hereford and Braford cattle. Journal of Animal Breeding and Genetics, v. 137, n. 5, p. 449-467, Sept. 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sul. |
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7. | | COMIN, H. B.; SOLLERO, B. P.; JUNQUEIRA, V. S.; CARDOSO, L. L.; HIGA, R. H.; CAETANO, A. R.; YOKOO, M. J. I.; CARDOSO, F. F. Controle de qualidade de genótipos e amostras utilizados na seleção genômica das raças Braford e Hereford In: SEMINÁRIO INTERINSTITUCIONAL DE ENSINO, PESQUISA E EXTENSÃO, 18.; MOSTRA DE INICIAÇÃO CIENTÍFICA, 16,; MOSTRA DE EXTENSÃO, 11., 2013, Cruz Alta. Ciência, conhecimento e sociedade de risco: anais. Cruz Alta: Unicruz, 2013.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sul. |
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10. | | JUNQUEIRA, V. S.; CARDOSO, F. F.; OLIVEIRA, M. M.; SOLLERO, B. P.; SILVA, F. F.; LOPES, P. S. Use of molecular markers to improve relationship information in the genetic evaluation of beef cattle tick resistance under pedigree-based models. Journal of Animal Breeding and Genetics, v. 134, n. 1, p. 14-26, Feb. 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sul. |
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12. | | JUNQUEIRA, V. S.; LOPES, P. S.; RESENDE, M. D. V. de; SILVA, F. F. e; LOURENÇO, D. A. L.; YOKOO, M. J. I.; CARDOSO, F. F. Impact of embryo transfer phenotypic records on large-scale beef cattle genetic evaluations. Revista Brasileira de Zootecnia, Viçosa, MG, v. 47, e20170033, 2018. 4 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Florestas; Embrapa Pecuária Sul. |
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13. | | JUNQUEIRA, V. S.; PEIXOTO, L. de A.; LAVIOLA, B. G.; BHERING, L. L.; MENDONCA, S.; COSTA, T. da S. A.; ANTONIASSI, R. Bayesian multi-trait analysis reveals a useful tool to increase oil concentration and to decrease toxicity in Jatropha curcas L. Plos One, v. 11, e0157038, 2016. DOI: 10.1371/journal.pone.015038Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agroenergia; Embrapa Agroindústria de Alimentos; Embrapa Recursos Genéticos e Biotecnologia. |
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14. | | OLIVEIRA, H. R.; SILVA, F. F.; SIQUEIRA, O. H. G. B. D.; SOUZA, N. O.; JUNQUEIRA, V. S.; RESENDE, M. D. V. de; BORQUIS, R. R. A.; RODRIGUES, M. T. Combining different functions to describe milk, fat, and protein yield in goats using Bayesian multiple-trait random regression models. Journal of Animal Science, v. 94 n. 5, p. 1865-1874, May 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Florestas. |
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17. | | REIMANN, F. A.; BOLIGON, A. A.; CAMPOS, G. S.; CARDOSO, L. L.; JUNQUEIRA, V. S.; CARDOSO, F. F. Genetic parameters and accuracy of traditional and genomic breeding values for eye pigmentation, hair coat and breed standard in Hereford and Braford cattle. Livestock Science, v. 213, p. 44-50, July 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Pecuária Sul. |
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18. | | SILVA, D. A. da; SILVA, F. F. e; VENTURA, H. T.; JUNQUEIRA, V. S.; SILVA, A. A. da; MOTA, R. R.; LOPES, P. S. Contemporary groups in the genetic evaluation of Nellore cattle using Bayesian inference. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n. 8, p. 643-651, fev. 2017. Título em português: Grupos de contemporâneos na avaliação genética de gado Nelore por inferência bayesiana.Biblioteca(s): Embrapa Unidades Centrais. |
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19. | | SIMÕES, M. R. S.; LEAL, J. J. B.; MINHO, A. P.; GULIAS GOMES, C. C.; MACNEIL, M. D.; COSTA, R. F.; JUNQUEIRA, V. S.; SCHMIDT, P. I.; CARDOSO, F. F.; BOLIGON, A. A.; YOKOO, M. J. I. Breeding objectives of Brangus cattle in Brazil. Journal of Animal Breeding Genetics, v. 37, n. 2, p. 177-188, Mar. 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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20. | | SIMÕES, M. R. S.; LEAL, J. J. B.; MINHO, A. P.; GULIAS GOMES, C. C.; MACNEIL, M. D.; COSTA, R. F.; JUNQUEIRA, V. S.; SCHMIDT, P. I.; CARDOSO, F. F.; BOLIGON, A. A.; YOKOO, M. J. I. Breeding objectives of Brangus cattle in Brazil. Journal of Animal Breeding and Genetics, v. 137, n. 2, p. 177-188, Mar. 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sul. |
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Registros recuperados : 22 | |
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